GSK3-beta Antibody

• Catalog Number: orb1527327
• Category: Antibodies
• Description: GSK3-beta Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Human, Mouse
• Immunogen: between 370 and 420
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:1,000 - 1:5,000; IP - Not recommended. Use rabbit anti-GSK3-beta antibody A302-049A.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: GSK3-beta
• UniProt ID: P49841
• NCBI: AAA66475.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: serine/threonine-protein kinase GSK3B; glycogen sy
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse GSK3-beta by western blot. Samples: Whole cell lysate from HeLa (5, 15, and 50 µg) , HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-GSK3-beta antibody orb1527327 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human GSK3-beta by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GSK3-beta antibody orb1527327 used for IP at 10 µg/mg lysate.