GSK3 beta antibody

• Catalog Number: orb1474591
• Category: Antibodies
• Description: Rabbit monoclonal antibody to GSK3 beta
• Species/Host: Rabbit
• Clonality: Monoclonal
• Tested applications: IH, WB
• Reactivity: Hamster, Human, Rat
• Immunogen: A synthetic peptide of human GSK3 beta
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100)
• Form/Appearance: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Conjugation: Unconjugated
• Target: GSK3B
• Entrez: 84027, 2932
• UniProt ID: P49841, P18266
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
• Alternative names: Glycogen synthase kinase-3 beta; GSK-3 beta; Serin
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of GSK3 beta expression in Jurkat (A), C6 (B), CHOK1 (C), Hela (D) whole cell lysates. (Predicted band size: 47 kD; Observed band size: 47 kD)

Immunohistochemical analysis of GSK3 beta staining in human cholangiocarcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.42). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.