GPX1 Antibody

• Catalog Number: orb1247967
• Category: Antibodies
• Description: GPX1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Porcine
• Immunogen: The immunogen for this antibody is: C-REALPAPSDDATA
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 22kDa band observed in Human Liver lysates (calculated MW of 22.1kDa according to NP_000572.2). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows staining of microglia. Recommended concentration: 3-5ug/ml. Paraffin embedded Human Liver. Recommended concentration: 3.75ug/ml. Frozen sections of Porcin Kidney shows staining of cytoplasm in PCT epithelial cells. Recommended concentration: 20ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: GPX1
• UniProt ID: P07203
• NCBI: NP_000572.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: glutathione peroxidase 1 , HGNC:4553, GPX1, GSHPX1
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 22kDa band observed in Human Liver lysates (calculated MW of 22.1kDa according to NP_000572.2). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows staining of microglia. Recommended concentration: 3-5ug/ml. Paraffin embedded Human Liver. Recommended concentration: 3.75ug/ml. Frozen sections of Porcin Kidney shows staining of cytoplasm in PCT epithelial cells. Recommended concentration: 20ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247967 (1 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247967 (3.8 ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247967 (20 ug/ml) staining of PFA-perfused cryosection of Porcin Kidney. Microwave antigen retrieval with citrate buffer pH 3, CY3-staining.

orb1247967 (3.75 ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.