GPX1 Antibody (monoclonal, 8B10)

• Catalog Number: orb527061
• Category: Antibodies
• Description: GPX1 Antibody (monoclonal, 8B10)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 8B10
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG2b
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human GPX1 (116-146aa EVNGAGAHPLFAFLREALPAPSDDATALMTD), different from the related mouse sequence by six amino acids and from the related rat sequence by five amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry/Immunofluorescence, 5μg/ml Flow Cytometry, 1-3μg/1x106 cells
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 22 kDa
• UniProt ID: P07203
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Glutathione peroxidase 1; GPx-1; GSHPx-1; Cellular
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U87 cells using anti-GPX1 antibody(Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of U251 cells using anti-GPX1 antibody (Blue line).Isotype control antibody (Green line) was mouse IgG .Unlabelled sample (Red line) was also used as a control.

WB:Lane 1:human placenta tissue;2:U-87 cell;3:THP-1 cell;4:U-937 cell;5:HepG2 cell;6:rat brain tissue;7:rat thymus tissue;8:rat lung tissue;9:rat liver tissue;10:mouse brain tissue;11:mouse thymus tissue;12:mouse lung tissue;13:mouse liver tissue.

IF analysis of GPX1 using anti-GPX1 antibody. GPX1 was detected in paraffin-embedded section of human colon cancer tissue.

IHC analysis of GPX1 using anti GPX1 antibody. GPX1 was detected in paraffin-embedded section of human lung cancer tissue.

IHC analysis of GPX1 using anti GPX1 antibody. GPX1 was detected in paraffin-embedded section of human mammary cancer tissue.