Glutathione Peroxidase 1/GPX1 Antibody

• Catalog Number: orb215957
• Category: Antibodies
• Description: Glutathione Peroxidase 1/GPX1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human GPX1 (116-146aa EVNGAGAHPLFAFLREALPAPSDDATALMTD), different from the related mouse sequence by six amino acids and from the related rat sequence by five amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat, By Heat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 22088 MW
• UniProt ID: P07203
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Glutathione peroxidase 1;GPx-1;GSHPx-1;1.11.1.9;Ce
• Note: For research use only
• Application notes: WB: The detection limit for GPX1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of GPX1 using anti-GPX1 antibody.Lane 1:human THP-1 cell;2:human HL-60 cell.3:human A549 cell.4:rat liver tissue;5:mouse liver tissue.

IHC analysis of GPX1 using anti-GPX1 antibody.GPX1 was detected in paraffin-embedded section of Mosue Pancreas Tissue.

IHC analysis of GPX1 using anti-GPX1 antibody.GPX1 was detected in paraffin-embedded section of Rat Pancreas Tissue.

IHC analysis of GPX1 using anti-GPX1 antibody.GPX1 was detected in paraffin-embedded section of Human Intestinal Cancer Tissue.