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    Glutamine Synthetase antibody

    Catalog Number: orb344215

    DispatchUsually dispatched within 5-10 working days
    $ 292.00
    Catalog Numberorb344215
    CategoryAntibodies
    DescriptionGlutamine Synthetase antibody
    Species/HostGoat
    ClonalityPolyclonal
    Tested applicationsELISA, IP, WB
    ReactivityBacteria
    IsotypeIgG
    ImmunogenGlutamine Synthetase [native from brevibacterium species]
    Dilution rangeELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
    Form/AppearanceLiquid (sterile filtered)
    PurityAnti-Glutamine Synthetase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutamine Synthetase [Microbial]. Cross reactivity against Glutamine Synthetase from other sources is unknown.
    ConjugationUnconjugated
    UniProt IDQ79VE3
    NCBICAG33988.1
    StorageStore vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesgoat anti-Glutamine Synthetase Antibody, Glutamine
    Read more...
    NoteFor research use only
    Application notesAnti-Glutamine Synthetase antibody has been tested by western blot and is suitable to be assayed against 1.0 µg of Glutamine Synthetase [Microbial] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:16,000 of the reconstitution concentration is suggested for this product. It is suitable for Immunohistochemistry also. Specific conditions for reactivity should be optimized by the end user.
    Expiration Date12 months from date of receipt.
    Glutamine Synthetase antibody

    Western Blot of Goat anti-Glutamine Synthetase antibody. Lane 1: Glutamine Synthetase Reduced. Lane 2: Glutamine Synthetase Non-Reduced. Load: ~1 ug of protein per lane. Primary antibody: Glutamine Synthetase antibody at 1:3000 for overnight at 4°C. Secondary antibody: Dylight 649 conjugated Donkey anti goat (1:10K) 1 hr RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 53.3 kDa, 56 kDa for Glutamine Synthetase. Other band(s): Glutamine Synthetase splice variants and isoforms.

    Glutamine Synthetase antibody

    Western blot of Goat Anti-Glutamine Synthetase primary antibody. Lane 1: Glutamine Synthetase. Lane 2: None. Load: 50 ng per lane. Primary antibody: Glutamine Synthetase antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase goat secondary antibody at 1:40000 for 30 min at RT. Blocking: orb348637 for 30 min at RT. Observed/Predicted size: 54 kDa, 54 kDa for Glutamine Synthetase (Brevibacterium). Other band(s): None.

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