Glutamine Synthetase Antibody / GLUL

• Catalog Number: orb2637135
• Category: Antibodies
• Description: Glutamine synthetase (Gl Syn) forms a homo-octamer that serves as a catalyst for the amination of glutamic acid to form glutamine. This enzyme is a marker for astrocytes, which serve as the primary site of conversion of glutamic acid to glutamine in the brain. Induction of glutamine synthetase is seen upon astrocyte cell contact with neurons. Elevated expression of glutamine synthetase in glial cells has been shown to protect neurons from degeneration due to excess glutamate. Glutamine synthetase is also present in the liver and is involved in nitrogen homeostasis. Overexpression of glutamine synthetase has been shown in primary liver cancers, indicating a potential role for glutamine synthetase in hepatocyte transformation.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GLUL/6604
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 50-250 was used as the immunogen for the GLUL antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This GLUL antibody is available for research use only.
• UniProt ID: P15104
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Glutamine Synthetase antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human Y79 cell lysate using GLUL antibody (clone GLUL/6604). Predicted molecular weight ~42 kDa.

Western blot testing of human liver tissue using GLUL antibody (clone GLUL/6604). Predicted molecular weight ~42 kDa.

IHC staining of FFPE human liver tissue with GLUL antibody (clone GLUL/6604) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free GLUL antibody (GLUL/6604) as confirmation of integrity and purity.