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GLI1 Rabbit Polyclonal Antibody (PE)

Catalog Number: orb496688

DispatchUsually dispatched within 2-3 weeks
$ 450.00
Catalog Numberorb496688
CategoryAntibodies
DescriptionGLI1 Rabbit Polyclonal Antibody (PE)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF
Predicted ReactivityBovine, Canine, Equine, Rabbit
ReactivityHuman, Mouse, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human GLI1/Zfp5 (601-700/1106aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeFlow-Cyt=1ug/Test, IF=1:100-500
ConjugationPE
TargetGLI1
StorageShipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesGli 1; Gli1; Gli-1; GLI; GLI Kruppel family member
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NoteFor research use only
Expiration Date12 months from date of receipt.
GLI1 Rabbit Polyclonal Antibody (PE)

Blank control: A549. Primary Antibody (green line): Rabbit Anti-GLI1 antibody (orb496688), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.