GLI1 Rabbit Polyclonal Antibody (BF555)

• Catalog Number: orb1585214
• Category: Antibodies
• Description: GLI1 Rabbit Polyclonal Antibody (BF555)
• Target: GLI1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: BF555
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Rabbit
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human GLI1/Zfp5 (601-700/1106aa)
• UniProt ID: P08151
• Tested applications: FC, IF
• Dilution range: Flow-Cyt=1ug/Test, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: Gli 1; Gli1; Gli-1; GLI; GLI Kruppel family member
• Research Area: DNA and RNA Research, Epigenetics and Nuclear Sign
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-GLI1 Antibody, conjugated (orb1585214) at 1:100 overnight at 4°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (Mouse uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-GLI1 Antibody, conjugated (orb1585214) at 1:100 overnight at 4°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.