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Catalog Number | orb234325 |
---|---|
Category | Antibodies |
Description | Galectin 3/LGALS3 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Mouse, Rat |
Isotype | Rabbit IgG |
Immunogen | E.coli-derived mouse Galectin 3 recombinant protein (Position: K153-I264). Mouse Galectin 3 shares 88% and 91% amino acid (aa) sequence identity with human and rat Galectin 3, respectively. |
Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
Dilution range | Western blot, 0.1-0.5μg/ml, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat, By Heat |
Form/Appearance | Lyophilized |
Conjugation | Unconjugated |
MW | 27515 MW |
UniProt ID | P16110 |
Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Alternative names | Galectin-3;Gal-3;35 kDa lectin;Carbohydrate-bindin Read more... |
Note | For research use only |
Application notes | WB: The detection limit for Galectin 3 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of Galectin 3/LGALS3 using anti-Galectin 3/LGALS3 antibody.Lane 1:rat lung tissue;2:rat stomach tissue;3:mouse lung tissue;4:mouse stomach tissue.
IHC analysis of Galectin 3/LGALS3 using anti-Galectin 3/LGALS3 antibody. Galectin 3/LGALS3 was detected in a paraffin-embedded section of mouse lung tissue.
IHC analysis of Galectin 3/LGALS3 using anti-Galectin 3/LGALS3 antibody. Galectin 3/LGALS3 was detected in a paraffin-embedded section of rat lung tissue.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC, WB | |
Canine, Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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