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Catalog Number | orb1247466 |
---|---|
Category | Antibodies |
Description | GAD2 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Predicted Reactivity | Canine, Mouse, Rat |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-TLEDNEERMSRLSK |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 65kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 65.4kDa according to NP_000809.1). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | GAD2 |
UniProt ID | Q05329 |
NCBI | NP_000809.1, NP_001127838.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | GAD2, GAD65, glutamate decarboxylase 2 (pancreatic Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 65kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 65.4kDa according to NP_000809.1). Recommended concentration: 0.5-1.5ug/ml. Primary incubation was 1 hour. |
Expiration Date | 12 months from date of receipt. |
orb1247466 (0.5 ug/ml) staining of Human Brain (Hippocampus) lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
orb1247466 (2.5 ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
orb1247466 (2.5 ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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