GAD2 Antibody

• Catalog Number: orb1270363
• Category: Antibodies
• Description: GAD2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Mouse, Porcine, Rat
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This GAD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-138 amino acids from the Central region of human GAD2.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For IF starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 65 kDa
• Target: GAD2
• UniProt ID: Q05329
• NCBI: Q05329
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Glutamate decarboxylase 2, 65 kDa glutamic acid de
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For IF starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysate from rat brain tissue lysate, using GAD2 Antibody at 1:1000.

Western blot analysis in Jurkat cell line lysates (35 ug/lane).

GAD2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Confocal immunofluorescent analysis of GAD2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).