Fumarase antibody

• Catalog Number: orb323143
• Category: Antibodies
• Description: Mouse monoclonal antibody to Fumarase
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Fumarase. The exact sequence is proprietary.
• Dilution range: WB: 1:1000-1:3000, IF/ICC: 1:100-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: FH
• Entrez: 14194, 2271
• UniProt ID: P97807, P14408, P07954
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Fumarate hydratase mitochondrial antibody, an
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Fumarase expression in 293T (A), HepG2 (B), Hela (C), mouse brain (D), rat brain (E) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 48 kD)

Immunofluorescent analysis of Fumarase staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).