FOXA1 Antibody

• Catalog Number: orb750047
• Category: Antibodies
• Description: The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1 (a and beta), HNF-3 (a, beta and gamma), HNF-4 (a and gamma), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Purity: Protein G affinity chromatography
• Immunogen: A human partial recombinant protein corresponding to amino acids 372-472 was used as the immunogen for this FOXA1 antibody.
• UniProt ID: P55317
• Tested applications: FACS, IF, IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-2ug/million cells
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the FOXA1 antibody to be titered up or down for optimal performance.
• Antibody Type: Primary Antibody
• Clone Number: FOXA1/1518
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Store the FOXA1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Hazard Information: This FOXA1 antibody is available for research use only.
• Note: For research use only
• Entrez: 3169

Western blot testing of human HeLa cell lysate with FOXA1 antibody (clone FOXA1/1518). Predicted molecular weight: ~49 kDa.

IHC testing of FFPE human prostate carcinoma with FOXA1 antibody (clone FOXA1/1518). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.

IHC testing of FFPE rat colon with FOXA1 antibody (clone FOXA1/1518). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.

Immunofluorescent staining of PFA-fixed human MCF7 cells with FOXA1 antibody (clone FOXA1/1518, green) and Phalloiden (red).

FACS testing of PFA-fixed human MCF-7 cells with FOXA1 antibody (clone FOXA1/1518); Red = isotype control, Blue = FOXA1 antibody.

SDS-PAGE Analysis of Purified, BSA-Free FOXA1 Antibody (clone FOXA1/1518). Confirmation of Integrity and Purity of the Antibody.