FOXA1 Antibody

• Catalog Number: orb2642011
• Category: Antibodies
• Description: The transcription factor Forkhead-box A1, also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1 (alpha and beta), HNF-3 (alpha, beta and gamma), HNF-4 (alpha and gamma), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. Its expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: FHBA1-1
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A human partial recombinant protein corresponding to amino acids 372-472 was used as the immunogen for this FOXA1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 2-4ug/ml
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This FOXA1 antibody is available for research use only.
• Entrez: 3169
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the FOXA1 antibody to be titered up or down for optimal performance.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human bladder carcinoma with FOXA1 antibody (clone FHBA1-1). Staining of FFPE tissue is enhanced by boiling sections in 10 mM Tris with 1 mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human prostate carcinoma with FOXA1 antibody (clone FHBA1-1). Staining of FFPE tissue is enhanced by boiling sections in 10 mM Tris with 1 mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.

Immunofluorescent staining of PFA-fixed human MCF7 cells with FOXA1 antibody (clone FHBA1-1, green) and Phalloiden (red).

FACS testing of PFA-fixed human MCF-7 cells with FOXA1 antibody (clone FHBA1-1); Red = isotype control, Blue = FOXA1 antibody.

Western blot testing of human HeLa cell lysate with FOXA1 antibody (clone FHBA1-1). Predicted molecular weight: ~49 kDa.