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Catalog Number | orb1577419 |
---|---|
Category | Antibodies |
Description | Fibronectin/FN1 Rabbit Polyclonal Antibody (BF647) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF |
Predicted Reactivity | Canine |
Reactivity | Bovine, Human, Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human Fibronectin/Ugl-Y3 (1201-1300/2386aa) |
Antibody Type | Primary Antibody |
Concentration | 1mg/ml |
Dilution range | IF=1:200-800 |
Conjugation | BF647 |
Target | FN1 |
Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
Alternative names | CIG; Cold insoluble globulin; DKFZp686F10164; DKFZ Read more... |
Background | _CLIENT_OVERRIDE_ |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Paraformaldehyde-fixed, paraffin embedded (Rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-Fibronectin Antibody, conjugated (orb1577419) 1:400, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.