Fibronectin Antibody

• Catalog Number: orb751105
• Category: Antibodies
• Description: Mouse monoclonal antibody to FN1
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: FN1/2948
• Tested applications: ELISA, IHC
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: Recombinant fragment (around aa 467-595) of human fibronectin protein (exact sequence is proprietary)
• Concentration: 200 μg/ml
• Dilution range: ELISA (For coating, order Ab without BSA),Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 220kDa (monomer); 440kDa (dimer)
• Target: Fn1
• Entrez: 2335
• UniProt ID: P02751
• Expression System: Bioreactor
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: Anti-Cold insoluble globulin (CIG) antibody, anti-
• Note: For research use only
• Application notes: Positive Control: SW156 cells.Humankidney tissue
• Expiration Date: 12 months from date of receipt.