Integrin Alpha V + Beta 1 Rabbit Polyclonal Antibody

• Catalog Number: orb13515
• Category: Antibodies
• Description: Integrin Alpha V + Beta 1 Rabbit Polyclonal Antibody
• Target: ITGAV
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Equine, Mouse, Porcine, Rat, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic Mixed peptides 915-948/1048(human Integrin αV)+ 21-70/798(human Integrin β1)
• UniProt ID: P06756
• RRID: AB_10748950
• MW: 114 kDa
• Tested applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg /test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Integrin alpha 5 beta 1; fibronectin receptor alph
• Research Area: Cardiovascula, Cardiovascular Research, Host-Virus
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Sato, Ryuta et al. Angiopoietin-like protein 2 induces androgen-independent and malignant behavior in human prostate cancer cells Oncol Rep, 33, 58-66 (2015)

PubMed: 25370833

Applications

IHC, WB

Reactivity

Human

Reichhart, Nadine et al. Rab27a GTPase modulates L-type Ca2+ channel function via interaction with the II-III linker of CaV1.3 subunit Cell Signal, 27, 2231-2240 (2015)

PubMed: 26235199

Applications

ICC

Reactivity

Hamster

Blank control (blue line): MCF7 (fixed with 70% methanol overnight at 4°C). Primary Antibody (green line): Rabbit Anti-Integrin Alpha V + Beta 1 antibody (orb13515), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/Test.

Sample: Lane 1: A549 (Human) Cell Lysate at 30 ug, Lane 2: U251 (Human) Cell Lysate at 30 ug, Lane 3: Huvec (Human) Cell Lysate at 30 ug, Lane 4: SH-SY5Y (Human) Cell Lysate at 30 ug, Lane 5: U2os (Human) Cell Lysate at 30 ug, Lane 6: MCF-7 (Human) Cell Lysate at 30 ug, Lane 7: Jurkat (Human) Cell Lysate at 30 ug, Primary: Anti-Integrin Alpha V + Beta 1 (orb13515) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 125 kD, Observed band size: 125 kD.