FGFR2 Antibody (N-term)

• Catalog Number: orb1788052
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: 7-37 aa
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:25, WB: 1:1000, IHC-P: 1:25, IHC-P: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 92025 Da
• Target: This FGFR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-37 amino acids from the N-terminal region of human FGFR2.
• UniProt ID: P21802
• NCBI: NP_075259.4, NP_075418.1, NP_001138388.1, NP_001138389.1, NP_001138390.1, NP_000132.3, NP_001138391.1, NP_001138387.1, NP_001138385.1, NP_001138386.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Fibroblast growth factor receptor 2, FGFR-2, K-sam
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded H. brain section using FGFR2 Antibody (N-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H. liver section using FGFR2 Antibody (N-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Fluorescent image of Hela cells stained with FGFR2 Antibody (N-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

All lanes: Anti-FGFR2 Antibody (R22) at 1:1000 dilution. Lane 1: Hela whole cell lysates. Lane 2: K562 whole cell lysates. Lane 3: MCF-7 whole cell lysates. Lane 4: T47D whole cell lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 92 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.