ERp57 (GRP58) antibody

• Catalog Number: orb147627
• Category: Antibodies
• Description: Mouse monoclonal to Erp57 (ATTO-488). ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface. ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus..
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Map.ERp57
• Tested applications: ICC, IHC, IP, WB
• Reactivity: Bovine, Canine, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat
• Isotype: IgG1
• Immunogen: Human recombinant ERp57 (Grp58)
• Concentration: 1 mg/ml
• Dilution range: WB (1:2000), IHC (1:100), ICC/IF (1:100)
• Conjugation: ATTO-488
• MW: 57kDa
• Target: ERp57
• Entrez: 2923
• UniProt ID: P30101
• NCBI: NP_005304.3
• Storage: Conjugated antibodies should be stored at 4°C
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
• Alternative names: ERp60 antibody, ERp61 antibody, Grp57 antibody, Gr
• Note: For research use only
• Application notes: 0.5 µg/ml of SMC-168 was sufficient for detection of ERp57 in 10 µg of heat shock Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.