ERK2 Antibody

• Catalog Number: orb1527304
• Category: Antibodies
• Description: ERK2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse
• Immunogen: between 310 and 360
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: ERK2
• UniProt ID: P28482
• NCBI: NP_002736.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: PRKM1; p38; p40; p41; p41mapk; P42MAPK; ERK2; PRKM
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse ERK2 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) , HEK293T (T; 50 µg) and mouse NIH 3T3 (M; 50µg) cells. Antibody: Affinity purified rabbit anti-ERK2 antibody orb1527304 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human ERK2 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ERK2 (Cat. No. orb1527304) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB