ERK1 Antibody

• Catalog Number: orb1527305
• Category: Antibodies
• Description: ERK1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: between 329 and 379
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: ERK1
• UniProt ID: P27361
• NCBI: NP_002737.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: HS44KDAP; MAPK 1; p44-MAPK; P44MAPK; p44-ERK1; P44
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human ERK1 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-ERK1 antibody orb1527305 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with exposure time of 30 seconds.

Detection of human ERK1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ERK1 antibody orb1527305 used for IP at 3 µg/mg lysate.

Detection of human ERK1 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-ERK1 (Cat. No. orb1527305) used at a dilution of 1: 200 (1µg/ml). Detection: DAB