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Catalog Number | orb1254424 |
---|---|
Category | Antibodies |
Description | ERCC2 Antibody |
Target | ERCC2 |
Clonality | Polyclonal |
Isotype | IgG |
Conjugation | Unconjugated |
Reactivity | Human, Rat |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Liquid |
Purification | Affinity purification |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ERCC2 (NP_001124339.1). |
UniProt ID | P18074 |
MW | Observed: 87kDa |
Tested applications | IF, WB |
Application notes | WB: 1:500 - 1:2000IF: 1:50 - 1:100 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | COFS2, EM9, TFIIH, TTD, TTD1, XPD, general transcr Read more... |
Note | For research use only |
Western blot analysis of extracts of various cell lines, using ERCC2 antibody (orb1254424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of HeLa cells using ERCC2 antibody (orb1254424). Blue: DAPI for nuclear staining.
ELISA, FC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |