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| Catalog Number | orb1254424 |
|---|---|
| Category | Antibodies |
| Description | ERCC2 Antibody |
| Target | ERCC2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Reactivity | Human, Rat |
| Form/Appearance | Liquid |
| Concentration | batch dependent |
| Buffer/Preservatives | Liquid |
| Purification | Affinity purification |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ERCC2 (NP_001124339.1). |
| UniProt ID | P18074 |
| MW | Observed: 87kDa |
| Tested applications | IF, WB |
| Application notes | WB: 1:500 - 1:2000IF: 1:50 - 1:100 |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | COFS2, EM9, TFIIH, TTD, TTD1, XPD, general transcr Read more... |
| Research Area | Cell Biology |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |

Western blot analysis of extracts of various cell lines, using ERCC2 antibody (orb1254424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of HeLa cells using ERCC2 antibody (orb1254424). Blue: DAPI for nuclear staining.
ELISA, FC, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |