ERCC2 Antibody

• Catalog Number: orb1254424
• Category: Antibodies
• Description: ERCC2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human ERCC2 (NP_001124339.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 87kDa
• Target: ERCC2
• UniProt ID: P18074
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: COFS2, EM9, TFIIH, TTD, TTD1, XPD, general transcr
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using ERCC2 antibody (orb1254424) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of HeLa cells using ERCC2 antibody (orb1254424). Blue: DAPI for nuclear staining.