ERAS Antibody

• Catalog Number: orb1268113
• Category: Antibodies
• Description: ERAS Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This ERAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-81 amino acids from the N-terminal region of human ERAS.
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 25 kDa
• Target: ERAS
• UniProt ID: Q7Z444
• NCBI: Q7Z444
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: GTPase ERas, E-Ras, Embryonic stem cell-expressed
• Note: For research use only
• Application notes: For IF starting dilution is: 1:100For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Fluorescent confocal image of SY5Y cells stained with ERAS (F66) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ERAS (F66) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ERAS immunoreactivity is localized to the plasma membrane of SY5Y cells.

Western blot analysis of ERAS Antibody (F66) in HL60 cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human brain tissue reacted with ERAS antibody (F66), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.