EpCAM antibody

• Catalog Number: orb555856
• Category: Antibodies
• Description: Mouse monoclonal antibody to EpCAM
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HEA125
• Tested applications: ELISA, FC, IF, IHC
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Viable HT-29 human colon carcinoma cell line
• Concentration: 1 mg/ml
• Dilution range: IHC on frozen sections !:200 to 1:500
• Conjugation: Unconjugated
• Hazard Information: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• UniProt ID: P16422
• Source: HEA125 is a mouse monoclonal IgG1 antibody derived by fusion of Ag8.653 mouse myeloma cells with splenocytes from a BALB/c mouse cells immunized with the HT-29 colon carcinoma cell line.
• Storage: Storage: The antibody may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product. Product Stability: For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product
• Buffer/Preservatives: ProtG affinity chromatography
• Note: For research use only
• Application notes: Positive Control: Human appendix, human kidney. Pre-Treatment: Pronase 0.1% or unmasking fluid C (Art.No. DE000). Incubation Time: 60 min at RT
• Expiration Date: 12 months from date of receipt.