EpCAM (intracellular domain) antibody

• Catalog Number: orb1294312
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EpCAM antibody (intracellular domain)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rabbit
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Intracellular domain corresponding to Human EpCAM
• Dilution range: Western Blot 1:1000–1:2000 Immunofluorescence 1:200–1:500 Immunohistochemistry (Paraffin) 1:200-1:300
• Conjugation: Unconjugated
• Target: EpCAM / CD326 (Intracellular domain)
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Cell : A549;DAPI : 1:300;Phalloidin : 1:300;EpCAM : 1:200;Anti-rabbit 488 : 1:500;NikonEpi-fluorescence(A-ken) 60x.

Immunofluorescence:cells were stained with anti-EpCAM antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Western blot analysis using Anti-EpCAM antibody at 1:800 dilution, lysates/proteins at 60 μg per lane.

Western blot analysis using Anti-EpCAM antibody at 1:800 dilution, lysates/proteins at 60 μg per lane.

Immunohistochemical analysis of paraffin embedded human cancer tissue labeling EpCAM with orb1294312 at 1/200 RT 1hr.

Immunohistochemical analysis of paraffin embedded human cancer tissue labeling EpCAM with orb1294312 at 1/200 RT 1hr.

Western blot analysis using Anti-EpCAM antibody at 1:1000 dilution, lysates at 40 μg per lane.