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EPCAM Antibody
Description
Images & Validation
−| Tested Applications | FC, IHC, WB |
|---|---|
| Dilution range | WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; FCM: 1/200 - 1/400; ELISA: 1/10000 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 7B5C10 |
| Immunogen | Purified recombinant fragment of human EPCAM (AA: 24-265) expressed in E. Coli. |
| Target | EPCAM |
| Molecular Weight | 35kDa |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody in PBS with 0.05% sodium azide. |
| Concentration | Approximately 1mg/ml (varies from batch to batch). Please inquire for precise concentration. |
| Disclaimer | For research use only |
Alternative Names
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Western blot analysis of A431 (Lane 1), MCF-7 (Lane 2) cell lysate using EPCAM antibody

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of EPCAM antibody

Flow cytometric analysis of A431 cells lysates using EPCAM antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded cervical cancer tissues using EPCAM antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded esophageal cancer tissues using EPCAM antibody

Western blot analysis of human EPCAM using EPCAM antibody

Western blot analysis of HEK293 (Lane 1) HEK293 (Lane 2) cell lysate using EPCAM antibody
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Protocol Information
EPCAM Antibody (orb197816)
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