EpCAM antibody

• Catalog Number: orb44756
• Category: Antibodies
• Description: Mouse Monoclonal antibody againts EpCAM
• Clonality: Monoclonal
• Clone Number: VU-1D9
• Tested applications: FC, ICC, IHC-Fr, IHC-P, IP, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Small cell lung carcinoma cell line H69.
• Concentration: 1 mg/ml
• Dilution range: Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/ml; positive control: colon epithelium, antigen retrieval: heat (sodium citrate) + trypsin. Immunocytochemistry: Recommended dilution: 4 μg/ml. Western blotting: Recommended dilution: 1-4 μg/ml, non-reducing conditions. Flow cytometry: Recommended dilution: 1-3 μg/ml. Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein in 1 ml lysate.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD326
• Entrez: 4072
• UniProt ID: P16422
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti Adenocarcinoma associated antigen antibody, a
• Note: For research use only
• Application notes: Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/ml; positive control: colon epithelium, antigen retrieval: heat (sodium citrate) + trypsin. Immunocytochemistry: Recommended dilution: 4 μg/ml. Western blotting: Recommended dilution: 1-4 μg/ml. Flow cytometry: Recommended dilution: 1-3 μg/ml. Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein in 1 ml lysate.
• Expiration Date: 12 months from date of receipt.

Separation of MCF-7 cells (red-filled) from SP2 cells (black-dashed) in flow cytometry analysis (surface staining) of cell lines stained using anti-human CD326 (VU-1D9) purified antibody (concentration in sample 6 µg/ml) GAM APC.

Flow cytometry surface staining pattern of MCF-7 cells stained using anti-human CD326 (VU-1D9) purified antibody (concentration in sample 6 µg/ml) GAM APC.

Immunohistochemistry staining of human prostate (paraffin sections) using anti-CD326 (VU-1D9).

Western blotting analysis of human CD326 using mouse monoclonal antibody VU-1D9 on lysates of MCF-7 cells and HEK293T cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. CD326 was detected around 40 kDa.