EPCAM Antibody

• Catalog Number: orb1274270
• Category: Antibodies
• Description: EPCAM Antibody
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rVU-1D9
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Small cell lung carcinoma cells were used as the immunogen for this recombinant Ep-CAM antibody.
• Concentration: 0.2 mg/mL
• Dilution range: Prediluted format: incubate for 30 min at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Ep-CAM antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: EPCAM
• UniProt ID: P16422
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Epithelial cell adhesion molecule, Ep-CAM, Adenoca
• Note: For research use only
• Application notes: Prediluted format: incubate for 30 min at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Ep-CAM antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of colon carcinoma with recombinant Ep-CAM antibody (clone rVU-1D9). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.