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EpCAM Antibody / Extracellular domain

Catalog Number: orb2640530

DispatchUsually dispatched within 5-10 working days
$ 780.00
Catalog Numberorb2640530
CategoryAntibodies
DescriptionEpCAM is frequently upregulated in carcinomas but is not expressed in cancers of non-epithelial origin. EpCAM expression is not limited to human colon carcinomas; in fact, EpCAM is expressed in a variety of human epithelial tissues, carcinomas, and progenitor and stem cells. [Wiki]
Species/HostRabbit
ClonalityRecombinant
Clone NumberEGP40/2041R
Tested applicationsIHC-P, WB
ReactivityCanine, Feline, Human
IsotypeRabbit IgG, kappa
ImmunogenA portion of amino acids 100-224 (extracellular domain) was used as the immunogen for the recombinant EpCAM antibody.
Antibody TypePrimary Antibody
Dilution rangeWestern blot:1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein A affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis recombinant EpCAM antibody is available for research use only.
UniProt IDP16422
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesTitering of the recombinant EpCAM antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
EpCAM Antibody / Extracellular domain

IHC testing of FFPE human thyroid carcinoma and recombinant EpCAM antibody (clone EGP40/2041R). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

EpCAM Antibody / Extracellular domain

IHC testing of FFPE human basal cell carcinoma and recombinant EpCAM antibody (clone EGP40/2041R). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

EpCAM Antibody / Extracellular domain

Western blot testing of human MCF7 cell lysate with recombinant EpCAM antibody (clone EGP40/2041R). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).

EpCAM Antibody / Extracellular domain

Western blot testing of human lung lysate with EpCAM antibody (clone EGP40/2041R). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).

EpCAM Antibody / Extracellular domain

SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (clone EGP40/2041R) as confirmation of integrity and purity.

EpCAM Antibody / Extracellular domain

IHC testing of FFPE dog bladder and recombinant EpCAM antibody (clone EGP40/2041R). HIER: steam sections in pH9 EDTA for 10-20 min.

EpCAM Antibody / Extracellular domain

IHC testing of FFPE cat small intestine and recombinant EpCAM antibody (clone EGP40/2041R). HIER: steam sections in pH9 EDTA for 10-20 min.

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