ENOA Antibody

• Catalog Number: orb1264536
• Category: Antibodies
• Description: ENOA Antibody
• Target: ENO1
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Monkey
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Immunogen: This ENOA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 405-434 amino acids from the C-terminal region of human ENOA.
• UniProt ID: P06733
• MW: 47 kDa
• Tested applications: FC, IF, IHC-P, WB
• Application notes: For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Alpha-enolase, 2-phospho-D-glycerate hydro-lyase,
• Note: For research use only
• NCBI: P06733

Western blot analysis in Jurkat, MCF-7, U251 cell line and mouse brain tissue lysates (35 ug/lane).

Flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis of ENOA Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

ENOA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.