EIF4E antibody

• Catalog Number: orb1474065
• Category: Antibodies
• Description: Mouse monoclonal antibody to EIF4E
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant fusion protein of human EIF4E. The exact sequence is proprietary.
• Dilution range: WB (1/1000 - 1/2000), IF/IC (1/10 - 1/50)
• Form/Appearance: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: EIF4E
• Entrez: 1977, 117045, 13684
• UniProt ID: P63074, P06730, P63073
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: EIF4EL1; EIF4F; Eukaryotic translation initiation
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of EIF4E expression in Hela (A), MCF7 (B), K562 (C), NIH3T3 (D), PC12 (E) whole cell lysates. (Predicted band size: 25 kD; Observed band size: 25 kD)

Immunofluorescent analysis of EIF4E staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).