EIF4E Antibody

• Catalog Number: orb1253729
• Category: Antibodies
• Description: EIF4E Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Immunogen: eIF4E (Phospho-Ser209) antibody was raised against a peptide sequence around phosphorylation site of serine 209 (S-G-S (p) -T-T) derived from Human eIF4E.
• Concentration: 1 mg/mL
• Dilution range: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 25 kDa
• Target: EIF4E
• UniProt ID: P06730
• NCBI: NP_001124150.1
• Storage: Store antibody at -20°C for up to one year.
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Alternative names: CBP, EIF4F, AUTS19, EIF4E1, EIF4EL1, BCL2L3, mRNA
• Note: For research use only
• Application notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysed extracts from HT29 cells untreated or treated with Anisomycin using eIF4E (Phospho-Ser209).

Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E (Phospho-Ser209). Bottom Image: Immunofluorescence staining of methanol-fixed MCF cells using eIF4E (Phospho-Ser209).