EIF2AK2 Antibody

• Catalog Number: orb1239891
• Category: Antibodies
• Description: EIF2AK2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-PKR antibody (orb1239891) was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human PKR. The immunogen is located within the last 50 amino acids of PKR.
• Concentration: 1 mg/mL
• Dilution range: WB: 1 μg/mL; IF: 20 μg/mL; IHC-P: 2.5-5 μg/mL.Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 62kDObserved: 68kD
• Target: EIF2AK2
• UniProt ID: P19525
• NCBI: AAP57628
• Storage: PKR antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: PKR Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: PKR Antibody: PKR, PRKR, EIF2AK1, PKR, Interferon-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western Blot Validation of PKR in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: PKR orb1239891 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: PKR orb1239891 (1 µg/mL), PKR orb1238780 (1 µg/mL, and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunofluorescence Validation of PKR in Mouse Lung. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling PKR with orb1239891 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Immunohistochemistry Validation of PKR in Rat Lung. Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-PKR antibody (orb1239891) at 2.5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of PKR in Mouse Lung. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse lung tissue labeling PKR with orb1239891 at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).

Immunohistochemistry Validation of PKR in Mouse Lung. Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-PKR antibody (orb1239891) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.