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Catalog Number | orb1238780 |
---|---|
Category | Antibodies |
Description | EIF2AK2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC-P, WB |
Predicted Reactivity | Mouse |
Reactivity | Human, Rat |
Isotype | IgG |
Immunogen | Anti-PKR antibody (orb1238780) was raised against a 14 amino acid synthetic peptide near the amino terminus of human PKR. The immunogen is located within the first 50 amino acids of PKR. |
Concentration | 1 mg/mL |
Dilution range | WB: 1-2 μg/mL; IHC-P: 5 μg/mL.Antibody validated: Western Blot in human and mouse samples and Immunohistochemistry in rat samples. All other applications and species not yet tested. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Predicted: 62kDObserved: 68kD |
Target | EIF2AK2 |
UniProt ID | P19525 |
NCBI | NP_002750 |
Storage | PKR antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | PKR Antibody is supplied in PBS containing 0.02% sodium azide. |
Alternative names | PKR Antibody: PKR, PRKR, EIF2AK1, PKR, Interferon- Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot Validation of PKR in MCF-7 Cells. Loading: 15 µg of lysates per lane. Antibodies: PKR orb1238780 (A: 1 µg/mL; B: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: PKR orb1239891 (1 µg/mL), PKR orb1238780 (1 µg/mL, and beta-actin (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation of PKR in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: PKR orb1238780 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Immunohistochemistry Validation of PKR in Rat Lung. Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-PKR antibody (orb1238780) at 5 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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