PKR (phospho-Thr446/451) antibody

• Catalog Number: orb6786
• Category: Tools
• Description: PKR (phospho-Thr446/451) antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthesised phosphopeptide derived from human PKR around the phosphorylation site of Thr446/451 (KR(p-T)RSKG(p-T)LR)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, ICC=1:100, Flow-Cyt=2ug/Test, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 62 kDa
• Target: Phospho-PKR (Thr446 + Thr451)
• UniProt ID: P19525
• Storage: Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol.
• Alternative names: double-stranded RNA-dependent Protein Kinase; inte
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraffin-embedded rat brain tissue with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). CLEC4A antibody at 1:200 dilution with overnight incubation at 4 degrees

Flow cytometric analysis of U251 cell lysate using PKR (phospho-Thr446/451) antibody

IF analysis of U251 cell lysate using PKR (phospho-Thr446/451) antibody

IHC-P image of rat brain tissue using PKR (phospho-Thr446/451) antibody

WB analysis of mouse spleen Lysate at 40 ug using PKR (phospho-Thr446/451) antibody

WB analysis of mouse liver Lysate at 40 ug using PKR (phospho-Thr446/451) antibody

WB analysis of Lane 1: Human HeLa cell lysates, Lane 2: Human K562 cell lysates, Lane 3: Human 293T cell lysates, Lane 4: Human A431 cell lysates using PKR (phospho-Thr446/451) antibody