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EIF2A Antibody / EIF2S1

Catalog Number: orb2636865

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb2636865
CategoryAntibodies
DescriptionThe initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex is composed of three subunits, designated eIF2a, eIF2band eIF2g (eukaryotic translation initiation factor 2 a, band g, respectively), all of which work in concert to form a ternary complex with GTP and tRNA in the early stages of protein synthesis. eIF2a, also known as EIF2S1 or EIF2, is a 315 amino acid subunit of the eukaryotic initiation complex that functions to bind tRNA to the 40S ribosomal subunit (in a GTP-dependent manner), thereby initiating translation. In addition, the phosphorylation state of eIF2a controls the rate of tRNA translation. When eIF2a is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2a is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation.
Species/HostMouse
ClonalityMonoclonal
Clone NumberPCRP-EIF2S1-1C11
Tested applicationsFACS
ReactivityHuman
IsotypeMouse IgG2a
ImmunogenRecombinant full-length human EIF2S1/EIF2A protein was used as the immunogen for the EIF2S1 antibody.
Antibody TypePrimary Antibody
Dilution rangeFlow cytometry: 1-2ug/million cells
PurityProtein A/G affinity
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Hazard InformationThis EIF2S1 antibody is available for research use only.
UniProt IDP05198
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the EIF2S1 antibody should be determined by the researcher.
Expiration Date12 months from date of receipt.
EIF2A Antibody / EIF2S1

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using EIF2S1 antibody (clone PCRP-EIF2S1-1C11). These results demonstrate the foremost specificity of the PCRP-EIF2S1-1C11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

EIF2A Antibody / EIF2S1

FACS staining of PFA-fixed human HeLa cells with EIF2S1 antibody (blue, clone PCRP-EIF2S1-1C11) and isotype control (red).

EIF2A Antibody / EIF2S1

SDS-PAGE analysis of purified, BSA-free EIF2S1 antibody (clone PCRP-EIF2S1-1C11) as confirmation of integrity and purity.

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