EIF2A Antibody / EIF2S1

• Catalog Number: orb2636865
• Category: Antibodies
• Description: The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex is composed of three subunits, designated eIF2a, eIF2band eIF2g (eukaryotic translation initiation factor 2 a, band g, respectively), all of which work in concert to form a ternary complex with GTP and tRNA in the early stages of protein synthesis. eIF2a, also known as EIF2S1 or EIF2, is a 315 amino acid subunit of the eukaryotic initiation complex that functions to bind tRNA to the 40S ribosomal subunit (in a GTP-dependent manner), thereby initiating translation. In addition, the phosphorylation state of eIF2a controls the rate of tRNA translation. When eIF2a is not phosphorylated, translation occurs at a normal rate. However, upon phosphorylation by one of several kinases, eIF2a is stabilized, thus preventing the GDP/GTP exchange reaction and slowing translation.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: Protein A/G affinity
• Immunogen: Recombinant full-length human EIF2S1/EIF2A protein was used as the immunogen for the EIF2S1 antibody.
• UniProt ID: P05198
• Tested applications: FACS
• Dilution range: Flow cytometry: 1-2ug/million cells
• Application notes: Optimal dilution of the EIF2S1 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: PCRP-EIF2S1-1C11
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This EIF2S1 antibody is available for research use only.
• Note: For research use only

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using EIF2S1 antibody (clone PCRP-EIF2S1-1C11). These results demonstrate the foremost specificity of the PCRP-EIF2S1-1C11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

FACS staining of PFA-fixed human HeLa cells with EIF2S1 antibody (blue, clone PCRP-EIF2S1-1C11) and isotype control (red).

SDS-PAGE analysis of purified, BSA-free EIF2S1 antibody (clone PCRP-EIF2S1-1C11) as confirmation of integrity and purity.