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EIF2A Antibody / EIF2S1
Catalog Number: orb1151617
| Catalog Number | orb1151617 |
|---|---|
| Category | Antibodies |
| Description | Mouse monoclonal antibody to EIF2A |
| Species/Host | Mouse |
| Clonality | Monoclonal |
| Clone Number | PCRP-EIF2S1-1C11 |
| Tested applications | FACS |
| Reactivity | Human |
| Isotype | Mouse IgG2a |
| Immunogen | Recombinant full-length human EIF2S1/EIF2A protein was used as the immunogen for the EIF2S1 antibody. |
| Antibody Type | Primary Antibody |
| Dilution range | Flow cytometry: 1-2ug/million cells |
| Purity | Protein A/G affinity |
| Conjugation | Unconjugated |
| Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide |
| Hazard Information | This EIF2S1 antibody is available for research use only. |
| UniProt ID | P05198 |
| Storage | Aliquot the EIF2S1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles. |
| Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide |
| Note | For research use only |
| Application notes | Optimal dilution of the EIF2S1 antibody should be determined by the researcher. |
| Expiration Date | 12 months from date of receipt. |
Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using EIF2S1 antibody (clone PCRP-EIF2S1-1C11). These results demonstrate the foremost specificity of the PCRP-EIF2S1-1C11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
FACS staining of PFA-fixed human HeLa cells with EIF2S1 antibody (blue, clone PCRP-EIF2S1-1C11) and isotype control (red).
SDS-PAGE analysis of purified, BSA-free EIF2S1 antibody (clone PCRP-EIF2S1-1C11) as confirmation of integrity and purity.
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