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EIF2A Antibody / EIF2S1

Catalog Number: orb1151651

DispatchUsually dispatched within 5-10 working days
$ 780.00
Catalog Numberorb1151651
CategoryAntibodies
DescriptionMouse monoclonal antibody to EIF2A
Species/HostMouse
ClonalityMonoclonal
Clone NumberPCRP-EIF2S1-1E2
Tested applicationsFACS, IF, WB
ReactivityHuman
IsotypeMouse IgG1
ImmunogenRecombinant full-length human EIF2S1 protein was used as the immunogen for the EIF2A antibody.
Antibody TypePrimary Antibody
Dilution rangeFlow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
PurityProtein A/G affinity
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Hazard InformationThis EIF2A antibody is available for research use only.
UniProt IDP05198
StorageAliquot the EIF2A antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the EIF2A antibody should be determined by the researcher.
Expiration Date12 months from date of receipt.
EIF2A Antibody / EIF2S1

Immunofluorescent staining of PFA-fixed human HeLa cells using EIF2A antibody (green, clone PCRP-EIF2S1-1E2) and phalloidin (red).

EIF2A Antibody / EIF2S1

Immunofluorescent staining of PFA-fixed human MCF-7 cells using EIF2A antibody (green, clone PCRP-EIF2S1-1E2).

EIF2A Antibody / EIF2S1

FACS staining of PFA-fixed human HeLa cells with EIF2A antibody (blue, clone PCRP-EIF2S1-1E2) and isotype control (red).

EIF2A Antibody / EIF2S1

SDS-PAGE analysis of purified, BSA-free EIF2A antibody (PCRP-EIF2S1-1E2) as confirmation of integrity and purity.

EIF2A Antibody / EIF2S1

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using EIF2A antibody (clone PCRP-EIF2S1-1E2). These results demonstrate the foremost specificity of the PCRP-EIF2S1-1E2 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

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