EGFR mutant (L858R)

• Catalog Number: orb153451
• Category: Antibodies
• Description: Mouse monoclonal antibody to EGFR mutant (L858R)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5G9B5
• Tested applications: ELISA, FC, IHC, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Purified recombinant fragment of human EGFR mutant (AA: 693-893) expressed in E. Coli.
• Concentration: 1mg/ml
• Dilution range: WB: 1/500 - 1/2000; IHC: 1/200 - 1/1000; RCM: 1/200 - 1/400; ELISA: 1/10000
• Conjugation: Unconjugated
• MW: 175kDa
• Entrez: 1956
• Storage: 4°C; -20°C for long term storage. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Purified antibody in PBS with 0.05% sodium azide
• Alternative names: ERBB; HER1; mENA; ERBB1; PIG61
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Line graph illustrates about the Ag-Ab reactions using different concentrations of antigen and serial dilutions of EGFR mutant antibody

Flow cytometric analysis of HepG2 cells lysates using EGFR mutant antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded cervical cancer tissues using EGFR mutant antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded rectum cancer tissues using EGFR mutant antibody

Western blot analysis of human EGFR mutant using EGFR mutant antibody

Western blot analysis of HEK293 (Lane 1) HEK293 (Lane 2) cell lysate using EGFR mutant antibody

Western blot analysis of SPC-A-1 (Lane 1), A549 (Lane 2), HepG2 (Lane 3) MCF-7 (Lane 4) cell lysate using EGFR mutant antibody