EGFR (Extracellular domain) antibody

• Catalog Number: orb1294279
• Category: Antibodies
• Description: Rabbit polyclonal antibody to EGFR antibody (Extracellular domain)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human EGFR
• Dilution range: Western Blot 1:1000 Immunofluorescence 1:200–1:500 Immunohistochemistry (Paraffin) 1:100-1:200
• Conjugation: Unconjugated
• Target: EGFR (Extracellular)
• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-EGFR antibody - at 1/1000 dilution, Lysates/proteins at 50 μg per lane. This blot was produced using a 5% SDS-PAGE. The gel was run at 140V for 50 minutes before being transferred onto a Nitrocellulose membrane at 18V for 60 minutes. The membrane was then blocked for an hour before being incubated with orb1294279 overnight at 4°C.