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Catalog Number | orb248329 |
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Category | Antibodies |
Description | This mAb is specific to HER2 / ErbB2 and shows minimal cross-reaction with other members of the family. HER2 is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. HER2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | HRB2/451 |
Tested applications | ELISA, FACS, IF |
Reactivity | Human |
Isotype | Mouse IgG1, kappa |
Immunogen | Recombinant human protein was used as the immunogen for this HER2 antibody. |
Dilution range | ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-4ug/ml |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This HER2 antibody is available for research use only. |
Entrez | 2064 |
Storage | Store the HER2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the mAb to be titered up or down for optimal performance. |
Expiration Date | 12 months from date of receipt. |
Immunohistochemical analysis of formalin-fixed and paraffin-embedded breast invasive carcinoma tissue using HER2 ErbB2 antibody
Immunohistochemical analysis of formalin-fixed and paraffin-embedded breast DCIS (ductal carcinoma in situ) tissue using HER2 ErbB2 antibody
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