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EGF Receptor / EGFR Antibody

Catalog Number: orb606575

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb606575
CategoryAntibodies
DescriptionThis MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.
ClonalityMonoclonal
Species/HostMouse
IsotypeMouse IgG2b, kappa
ConjugationUnconjugated
ReactivityHuman
ImmunogenA portion of amino acids 344-492 from the human protein was used as the immunogen for the EGFR antibody.
UniProt IDP00533
Tested applicationsIHC-P, WB
Dilution rangeImmunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Application notesTitering of the EGFR antibody may be required for optimal performance.
Antibody TypePrimary Antibody
Clone NumberGFR/2341
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Hazard InformationThis EGFR antibody is available for research use only.
NoteFor research use only
EGF Receptor / EGFR Antibody

Western blot testing of A431 lysate using EGFR antibody (clone GFR/2341). Expected molecular weight: 134-180 kDa depending on glycosylation level.

EGF Receptor / EGFR Antibody

IHC testing of FFPE human placenta with EGFR antibody (clone GFR/2341). Required HIER: boiling tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

EGF Receptor / EGFR Antibody

IHC testing of FFPE human lung squamous cell carcinoma with EGFR antibody (clone GFR/2341). Required HIER: boiling tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min.

EGF Receptor / EGFR Antibody

SDS-PAGE analysis of purified, BSA-free EGFR antibody (clone GFR/2341) as confirmation of integrity and purity.

EGF Receptor / EGFR Antibody

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using EGFR antibody (clone GFR/2341). These results demonstrate the foremost specificity of the GFR/2341 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

  • EGF Receptor / EGFR Antibody [orb606515]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg, 100 μg
  • EGF Receptor / EGFR Antibody [orb699575]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg, 100 μg
  • EGF Receptor / EGFR Antibody [orb749349]

    FACS,  IF

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg, 100 μg
  • EGF Receptor / EGFR Antibody [orb750037]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg, 100 μg
  • EGF Receptor / EGFR Antibody [orb606695]

    IHC-P,  WB

    Human

    Rabbit

    Recombinant

    Unconjugated

    20 μg, 100 μg