Cart summary

You have no items in your shopping cart.

EGF Receptor / EGFR Antibody

Catalog Number: orb606515

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb606515
CategoryAntibodies
DescriptionReceptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, amphiregulin, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. [UniProt]
ClonalityMonoclonal
Species/HostMouse
IsotypeMouse IgG1, kappa
ConjugationUnconjugated
ReactivityHuman
ImmunogenPurified recombinant human EGFR was used as the immunogen for the EGF Receptor antibody.
UniProt IDP00533
Tested applicationsIHC-P, WB
Dilution rangeImmunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Application notesTitering of the EGF Receptor antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Antibody TypePrimary Antibody
Clone NumberGFR/1667
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Hazard InformationThis EGF Receptor antibody is available for research use only.
NoteFor research use only
EGF Receptor / EGFR Antibody

IHC testing of FFPE human bladder with EGF Receptor antibody (clone GFR/1667). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

EGF Receptor / EGFR Antibody

IHC testing of FFPE human placenta with EGF Receptor antibody (clone GFR/1667). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

EGF Receptor / EGFR Antibody

IHC testing of FFPE human lung squamous cell carcinoma with EGF Receptor antibody (clone GFR/1667). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

EGF Receptor / EGFR Antibody

Western blot testing of A431 lysate using EGFR antibody (clone GFR/1667). Expected molecular weight: 134-180 kDa depending on glycosylation level.

EGF Receptor / EGFR Antibody

SDS-PAGE analysis of purified, BSA-free EGF Receptor antibody (clone GFR/1667) as confirmation of integrity and purity.

  • EGF Receptor / EGFR Antibody [orb606575]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg, 100 μg
  • EGF Receptor / EGFR Antibody [orb699575]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg, 100 μg
  • EGF Receptor / EGFR Antibody [orb749349]

    FACS,  IF

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg, 100 μg
  • EGF Receptor / EGFR Antibody [orb750037]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg, 100 μg
  • EGF Receptor / EGFR Antibody [orb606695]

    IHC-P,  WB

    Human

    Rabbit

    Recombinant

    Unconjugated

    20 μg, 100 μg