EGF R Antibody

• Catalog Number: orb1252778
• Category: Antibodies
• Description: EGF R Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: GFR450
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Isotype: IgG2a, kappa
• Immunogen: The immunizing protein for this antibody was recombinant human EGFR.
• Concentration: 0.2 mg/mL
• Dilution range: IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Flow Cytometry: 0.5-1 ug/million cells in 0.1mlIF: 1-2 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EGFR antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: EGF R
• UniProt ID: P00533
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Epidermal growth factor receptor, Proto-oncogene c
• Note: For research use only
• Application notes: IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Flow Cytometry: 0.5-1 ug/million cells in 0.1mlIF: 1-2 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EGFR antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
• Expiration Date: 12 months from date of receipt.

IHC testing of squamous cell carcinoma stained with EGFR antibody (GFR450).

FACS testing of A431 cells with isotype control (gray) and EGFR antibody (green).

Immunofluorescent staining of A431 cells with Alexa Fluor conjugated EGFR antibody (green) and DAPI (nuclear, blue).

SDS-PAGE Analysis of Purified, BSA-Free EGFR Antibody (clone GFR450). Confirmation of Integrity and Purity of the Antibody.