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Catalog Number | orb1252103 |
---|---|
Category | Antibodies |
Description | EGF R Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | ERB59-1 |
Tested applications | FC, IF, IHC |
Reactivity | Human |
Isotype | IgG2a, kappa |
Immunogen | Recombinant human protein was used as the immunogen for this EGFR antibody. |
Concentration | 0.2 mg/mL |
Dilution range | IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EGFR antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | EGF R |
UniProt ID | P00533 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | Epidermal growth factor receptor, Proto-oncogene c Read more... |
Note | For research use only |
Application notes | IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EGFR antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. |
Expiration Date | 12 months from date of receipt. |
Immunofluorescent testing of A431 cells and Alexa Fluor conjugated EGFR antibody (green) with DAPI nuclear stain (blue).
IHC testing of lung squamous cell carcinoma stained with EGFR antibody (ERB59-1).
FACS testing of A431 cells with isotype control (gray) and EGFR antibody (green).
FC, IF, IHC-P, WB | |
Canine, Mouse, Porcine | |
Human, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
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