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Catalog Number | orb704502 |
---|---|
Category | Antibodies |
Description | EGFR antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from human EGFR |
Concentration | 1mg/ml |
Dilution range | WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC=1:50-200, ELISA=1:5000-10000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 175 kDa |
Target | EGFR |
UniProt ID | P00533 |
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4°C. |
Buffer/Preservatives | 0.01M TBS(pH7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% Glycerol. |
Alternative names | Avian erythroblastic leukemia viral (v erb b) onco Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
IHC-P analysis of rat placenta using EGFR Polyclonal Antibody at 1:200 dilution.
IHC-P analysis of human gastric carcinoma using EGFR Polyclonal Antibody at 1:200 dilution.
Western blot:Lane 1:Hela (Human) Cell;Lane 2:A549 (Human) Cell;Lane 3:U251 (Human) Cell;Lane 4:U87MG (Human) Cell;Primary:Anti-EGFR at 1:1000 dilution.
ELISA, ICC, IF, IHC-P, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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