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Catalog Number | orb1527963 |
---|---|
Category | Antibodies |
Description | eEF2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse |
Immunogen | between 808 and 858 |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | eEF2 |
UniProt ID | P13639 |
NCBI | NP_001952.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | epididymis secretory sperm binding protein; polype Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human and mouse eEF2 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eEF2 antibody orb1527963 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Detection of human and mouse eEF2 by immunohistochemistry.Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-eEF2 (Cat. No. orb1527963) used at a dilution of 1: 1, 000 (0.2µg/ml). Detection: DAB
FC, ICC, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Monkey, Mouse, Rat, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
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