eEF2 Antibody

• Catalog Number: orb1527962
• Category: Antibodies
• Description: eEF2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse
• Immunogen: between 808 and 858
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: eEF2
• UniProt ID: P13639
• NCBI: NP_001952.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: epididymis secretory sperm binding protein; polype
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse eEF2 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eEF2 antibody orb1527962 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of human and mouse eEF2 by immunohistochemistry.Sample: FFPE section of human breast carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-eEF2 (Cat. No. orb1527962) used at a dilution of 1: 1, 000 (0.2µg/ml). Detection: DAB