E2F6 antibody

• Catalog Number: orb213868
• Category: Antibodies
• Description: Rabbit polyclonal antibody to E2F6
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IH, IP, WB
• Reactivity: Human, Mouse, Porcine, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human E2F6. The exact sequence is proprietary.
• Dilution range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, ChIP: 1-100-1-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: E2F6
• Entrez: 1876, 50496
• UniProt ID: O75461, O54917
• Source: Rabbit
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Transcription factor E2F6 antibody, anti E2F-
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of E2F6 expression in PC12 (A), A2780 (B), HEK293T (C), HCT116 (D) whole cell lysates. (Predicted band size: 31 kD; Observed band size: 35 kD)

Immunohistochemical analysis of E2F6 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

ChIP analysis of Mouse ES cell nuclear lysate, incubated for 16 hours at 4°C. Cross-linking (X-ChIP) using formaldehyde for 10 minutes. Positive control: Tuba3. Negative Control: Beta-Actin. Detection step: Real-time PCR.